Identification of Aerobic Salivary Microorganisms in Patients With Oral Squamous Cell Carcinoma Using MALDI‐TOF MS: Preliminary Findings From a Pilot Study
ABSTRACT Rationale Oral squamous cell carcinoma (OSCC) is a multifactorial disease, and emerging evidence links the oral microbiome to its development. Rapid, noninvasive identification of salivary microorganisms may offer novel diagnostic and prognostic insights into oral cancer. Methods Salivary microbiota from OSCC patients and healthy individuals were analyzed using MALDI Biotyper. Saliva samples were cultured, and microbial identification was performed based on protein spectral profiles using an UltrafleXtreme MALDI‐TOF mass spectrometer. Results Thirteen OSCC patients (mean age 55 ± 11 years; 69% male) and nineteen healthy controls (mean age 55 ± 10 years; 79% male) were analyzed. Distinct microbial profiles were observed in OSCC patients, including pathogenic species previously associated with carcinogenesis, suggesting potential biomarkers for oral cancer. Conclusions The MALDI Biotyper is an effective, noninvasive tool for identifying salivary microbiota. Its application may support early diagnosis and prognosis of OSCC, reinforcing the significance of the oral microbiome in cancer etiology.
Citação
@online{silva_moreira,_milena2025,
author = {Silva~Moreira, Milena, Da and Giovanna , Schmitz and
Sá~Alves, Mariana, De and Maria~Anita , Mendes and
Mônica~Ghislaine~Oliveira , Alves and Levy~Anderson~César , Alves
and Meriellen , Dias and Celso~Muller , Bandeira and
Gabrielle~Luana~Jimenez~Teodoro , Nepomuceno and Silva~Martinho,
Herculano, Da and Janete~Dias , Almeida},
title = {Identification of Aerobic Salivary Microorganisms in Patients
With Oral Squamous Cell Carcinoma Using MALDI‐TOF MS: Preliminary
Findings From a Pilot Study},
volume = {39},
number = {15},
date = {2025-08-15},
doi = {10.1002/rcm.10063},
langid = {pt-BR},
abstract = {ABSTRACT Rationale Oral squamous cell carcinoma (OSCC) is
a multifactorial disease, and emerging evidence links the oral
microbiome to its development. Rapid, noninvasive identification of
salivary microorganisms may offer novel diagnostic and prognostic
insights into oral cancer. Methods Salivary microbiota from OSCC
patients and healthy individuals were analyzed using MALDI Biotyper.
Saliva samples were cultured, and microbial identification was
performed based on protein spectral profiles using an UltrafleXtreme
MALDI‐TOF mass spectrometer. Results Thirteen OSCC patients (mean
age 55 ± 11 years; 69\% male) and nineteen healthy controls (mean
age 55 ± 10 years; 79\% male) were analyzed. Distinct microbial
profiles were observed in OSCC patients, including pathogenic
species previously associated with carcinogenesis, suggesting
potential biomarkers for oral cancer. Conclusions The MALDI Biotyper
is an effective, noninvasive tool for identifying salivary
microbiota. Its application may support early diagnosis and
prognosis of OSCC, reinforcing the significance of the oral
microbiome in cancer etiology.}
}